实验技术与管理

为进一步增强学科间的交叉与渗透,提高学生灵活运用多学科知识与方法的能力,对质粒DNA提取及电泳检测实验进行改革。在改进经典碱裂解法提取质粒DNA、琼脂糖凝胶电泳检测质粒DNA的基础上,新增试剂盒方法提取质粒DNA、超微量分光光度计检测质粒DNA以及快速单酶切鉴定质粒DNA等实验内容。改革后的实验不仅促进经典与现代技术、方法的有机结合,而且增强学科间的相互融合,取得了良好的教学效果。

In order to further enhance the intercross and penetration of different disciplines and improve the students' ability to use the multi-disciplinary knowledge and methods flexibly,the reform on the experiment of plasmid DNA extraction and electrophoresis detection is carried out.Based on the extraction of plasmid DNA by the improved classical alkaline spitting method and the detection of plasmid DNA by agarose gelelectrophoresis,the experimental contents are newly increased such as the extraction of plasmid DNA by the new kit method,the detection of plasmid DNA by ultra micro-spectrophotometer,rapid single enzyme digestion identification of plasmid DNA,etc.After the reform,the experiment not only promotes the combination of classical technologies and methods with the modern ones,but also enhances the mutual integration of disciplines,achieving good teaching effect.